mirna microarray analysis (Macrogen)
90
Structured Review
Macrogen
mirna microarray analysis
Mirna Microarray Analysis, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mirna microarray analysis/product/Macrogen
Average 90 stars, based on 1 article reviews
Mirna Microarray Analysis, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mirna microarray analysis/product/Macrogen
Average 90 stars, based on 1 article reviews
mirna microarray analysis - by Bioz Stars,
2026-04
90/100 stars
Images
1) Product Images from "Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization"
Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
Journal: Cell Communication and Signaling : CCS
doi: 10.1186/s12964-024-01570-5
Figure Legend Snippet: MiR-6794-5p is upregulated within Bcl-w-derived exosomes. A Heatmap showing expressed exosomal miRNAs between vector and Bcl-w. B A549 cells were transfected with the negative control (NC), miR-1343-5p, miR-2861, miR-6794-5p, and miR-122-5p mimics, respectively. The mRNA expression levels of Twist, Zeb1, N-cad, and Oct4 by each miRNA were measured by qRT-PCR. The values were normalized to GAPDH. C Expression level of miR-6794-5p in exosomes isolated from conditioned media from Bcl-w-overexpressing A549 cells. D miR-6794-5p expression levels in Bcl-w overexpressing U251 and A549 cells. E The expression of miR-6794-5p in plasma of normal and patients with lung cancer (normal, n = 29; lung cancer, n = 24) was analyzed by qRT-PCR. The values were normalized to U6. Data are presented as mean ± SD after triplicate. * P < 0.05; ** P <0.01; *** P <0.001. Student's t-test
Techniques Used: Derivative Assay, Plasmid Preparation, Transfection, Negative Control, Expressing, Quantitative RT-PCR, Isolation, Clinical Proteomics
Figure Legend Snippet: miR-6794-5p directly inhibits the expression of SOCS1. A Venn diagram indicated target candidate genes of miR-6794-5p using TargetScan and miRWalk, which are miRNA target prediction sites. B, C After overexpression of the miR-6794-5p mimic in U251 ( B ) and A549 ( C ) cells, the mRNA expression of each of the candidate genes was confirmed by qRT-PCR. The values were normalized to GAPDH. D After miR-6794-5p was overexpressed in both cells, the level of SOCS1 protein was confirmed by Western blot analysis. β-actin was used for normalization. The experiment was repeated with triplicates and representative Western blotting images are shown. E, F Dual luciferase activity was examined after A549 cells were co-transfected with wild-type (WT) or mutant (Mut) vectors of the SOCS1 3’UTR in the presence or absence of the miR-6794-5p mimic, respectively. G Ago2-RNA immunoprecipitation (Ago2-IP) assay was performed in negative control (NC) or miR-6794-5p overexpressed A549 cells, and SOCS1 enrichment was confirmed by qRT-PCR. The values were normalized to GAPDH. H The mRNA expression of SOCS1 in plasma of normal and patients with lung cancer (normal, n = 23; lung cancer, n = 23) was analyzed by qRT-PCR. I Kaplan-Meier plots were used to compare survival rates between normal groups and lung adenocarcinoma patients. All data are presented as the mean ± S.D. after triplicate. * P < 0.05; ** P < 0.01; *** P < 0.001. Student’s t-test
Techniques Used: Expressing, Over Expression, Quantitative RT-PCR, Western Blot, Luciferase, Activity Assay, Transfection, Mutagenesis, RNA Immunoprecipitation, Negative Control, Clinical Proteomics
